The Mu Three-Site Synapse
نویسندگان
چکیده
منابع مشابه
Three-Site Synapsis during Mu DNA Transposition: A Critical Intermediate Preceding Engagement of the Active Site
The chemical steps of bacteriophage Mu DNA transposition take place within a higher order nucleoprotein structure. We describe a novel intermediate that precedes the previously characterized transpososomes and directly demonstrates the interaction of a distant enhancer element with recombination regions. The transpositional enhancer interacts with the Mu left and right ends to form a three-site...
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The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108...
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Transmitter concentration at a three-dimensional synapse. J. Neurophysiol. 80: 3163-3172, 1998. At intensities from starlight to 1000-fold brighter, the mammalian rod synapse transmits a binary signal, the capture of 0 or 1 photon. Zero is signified by tonic exocytosis, and 1 is signified by a brief pause. The synapse is three dimensional: vesicles discharge at the apex of a deep cleft created ...
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A $mu$-way $(v,k,t)$ $trade$ of volume $m$ consists of $mu$ disjoint collections $T_1$, $T_2, dots T_{mu}$, each of $m$ blocks, such that for every $t$-subset of $v$-set $V$ the number of blocks containing this t-subset is the same in each $T_i (1leq i leq mu)$. In other words any pair of collections ${T_i,T_j}$, $1leq i< j leq mu$ is a $(v,k,t)$ trade of volume $m$. In th...
متن کاملThe phiX174-type primosome promotes replisome assembly at the site of recombination in bacteriophage Mu transposition.
Initiation of Escherichia coli DNA synthesis primed by homologous recombination is believed to require the phiX174-type primosome, a mobile priming apparatus assembled without the initiator protein DnaA. We show that this primosome plays an essential role in bacteriophage Mu DNA replication by transposition. Upon promoting transfer of Mu ends to target DNA, the Mu transpososome undergoes transi...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2002
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(02)00596-8